In the modern era of urbanization, industrialization and polluted environment in general and also in relation to occupation areas have contributed significantly on human health. The trace elements present in human body plays a vital role in normal functioning, due to external environment, food habits etc. the levels of trace elements are significantly affected, in Ajmer region of Rajasthan past bio-monitoring studies of hairs, nail and scalp have shown significant variation in trace element levels. In correlation with the elemental load on body; correlated studies on blood, urine, serum etc. are also needed, advanced studies taking autopsy samples of major organs can also suggest the level of impact happening on human body.