The present study was aimed to carry out the detailed phytochemical analysis of the leaves of Pistia stratiotes. Qualitative phytochemical screening of the aqueous and methanolic extracts of the leaves revealed the presence of many components such as aroma agent, vitamin E, vitamin K, vitamin D, carcinogens, steroid hormones and cholesterol. GC-MS analysis was also carried out to detect the phytoconstituents present in the methanolic extract of the leaves P. stratiotes.