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VOL. 8, ISSUE 1 (2023)
Antibacterial activities of ethyl asetat fraction Sargassum Polycystum on the growth of Staphylococcus Aureus ATCC 25923
Authors
Pramudita Riwanti, Mahmiah, Nurul Fawaidah
Abstract
Secondary metabolites found in Sargassum
polycystum include flavonoids, alkaloids, saponins, steroids, and
triterpenoids, all of which have antibacterial properties. Staphylococcus
aureus is one of the pathogenic bacteria that can infect humans. The aim of
this work was to establish the antibacterial activity of the ethyl acetate
fraction against Staphylococcus aureus bacteria and to determine the
secondary metabolite compounds in Sargassum polycystum ethyl acetate
fraction. The liquid-liquid partition method is used to obtain the ethyl
acetate fraction. The diffusion method (Well Diffusion Method) is the
research technique used to study antibacterial activity. According to the
results of the phytochemical screening, Sargassum polycystum positive
contained steroids in the ethyl acetate fraction. The antibacterial activity
study's findings revealed readings of the inhibitory negative control zone
diameter of 0.00 mm and the positive control diameter of 34.46 mm. Five
different concentrations were used in the control test: 0.2, 0.4, 0.8, 1, 2,
and 2% (b/v). The average measurement findings are less than 5 mm. It is
believed that secondary steroid metabolites are in charge of Staphylococcus
aureus's antibacterial action. The Sargassum polycystum's ethyl
acetate fraction has a weak antibacterial activity, that is less than 5 mm.
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Pages:13-16
How to cite this article:
Pramudita Riwanti, Mahmiah, Nurul Fawaidah "Antibacterial activities of ethyl asetat fraction <i>Sargassum Polycystum</i> on the growth of <i>Staphylococcus Aureus</i> ATCC 25923". National Journal of Multidisciplinary Research and Development, Vol 8, Issue 1, 2023, Pages 13-16
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